Conversion of phenylalanine into tyrosine by portulaca callus.
نویسنده
چکیده
The incorporation of [(14)C]phenylalanine and [1,6-(14)C]shikimic acid into tyrosine was investigated in the callus of Portulaca grandiflora, var. JR (L.). By inhibiting phenylalanine with 1-alpha-aminooxy-beta-phenyl-propionic acid and tyrosinase with 1-cysteine-HCl and hydrazine-hydrate, the possible synthesis of tyrosine from phenylalanine was demonstrated. Tetrahydropterine sulfate was an effective activator of this pathway and tyrosine accumulation via 4-hydroxy-prephenic acid is regulated by feedback inhibition. l-alpha-Amminooxy-beta-phenylpropionic acid inhibits both phenylalanine ammonia-lyase and the production of phenylalanine from prephenic acid.
منابع مشابه
Cell Suspension Cultures of Portulaca grandiflora as Potent Catalysts for Biotransformation of L-Tyrosine into L-DOPA, an Anti-Parkinson’s Drug
3,4-Dihydroxy L-phenylalanine (L-DOPA) is considered a potent drug for the treatment of Parkinson disease, a neurologic disorder. Enantiomerically pure L-DOPA is produced from L-tyrosine in a single-step biotransformation process using callus cultures of the plant Portulaca grandifloraHook (Portulacaceae). Callus cultures were induced in MS medium provided with growth regulators such as benzyla...
متن کاملTyrosine and Phenylalanine Ammonia Lyase Activities during Shoot Initiation in Tobacco Callus Cultures.
Both phenylalanine ammonia lyase and tyrosine ammonia lyase were detected in tobacco (Nicotiana tabacum L. Wisconsin 38) callus. The enzymes were separated from each other by Sephadex G-200 column chromatography. Increased activity of tyrosine ammonia lyase was observed during culture of tobacco callus under shoot-forming conditions, while activity of phenylalanine ammonia lyase increased durin...
متن کاملComparison of phenylalanine and tyrosine in urine of patients with vitiligo and normal subjects
Amino acids are one of the most necessary substances in intracellular metabolic processes.aromatic aminoacids such as phenylalanine and tyrosine are precursor of melanin so are important materials for the skin pigmentation.metabolic disorders in the melanin synthesis causes milky macules on the skin of patients,known as vitiligo.these patients are hypersensitive to sunlight and consequently at ...
متن کاملCallus induction in Portulaca oleracea L. by different hormone concentrations and explant types
Purslane (Portulaca oleracea L.) contains valuable secondary metabolites such as Dopamin, Noradrenaline and Omega-3. This plant is used in various medicinal, food and hygienic industries as well as the treatment of different diseases such as diabetes, heart disease and pain relief. Callus induced from medicinal plants are used to increase the production of secondary metabolities in cell suspens...
متن کاملCallus Induction and Application of Methyle Jasmonate to Increase Dopamine in Portulaca oleracea L.
Purslane (Portulaca oleracea L.) contains valuable ingredients such as dopamine, noradrinalin and omega-3. The increase of secondary metabolites by elicitors is widely used in tissue culture of medicinal plants. The aim of this study was to assess effect of plant growth regulators on callus induction and amount of dopamine in callus tissue of purslane under the influence of methyl jasmonate as ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 68 2 شماره
صفحات -
تاریخ انتشار 1981